Glycopolymer Microarrays with Sub‐Femtomolar Avidity for Glycan Binding Proteins Prepared by Grafted‐To/Grafted‐From Photopolymerizations

Abstract We report a novel glycan array architecture that binds the mannose‐specific binding protein, concanavalin A (ConA), with sub‐femtomolar avidity. new radical photopolymerization developed specifically for this application combines grafted‐from thiol–(meth)acrylate polymerization thiol–ene chemistry to graft glycans growing polymer brushes. The propagation of brushes was studied by carrying out grafted‐to/grafted‐from (GTGFRP) at >400 different conditions using hypersurface photolithography, printing strategy substantially accelerates reaction discovery and optimization on surfaces. effect brush height grafting density mannosides ConA systematically, we found multivalent cooperative account unprecedented sensitivity GTGFRP This study further demonstrates ease which can be tailored an as result advantages photolithography.